Tris-HCl Buffer, pH 7.5 [1M] - 1000 ml
Produced under the highest industry standards to ensure superior results.
Tris-HCl Buffer, pH 7.5 [1M] - 1000 ml
Articolo #: 88027277

Tris-HCl Buffer, pH 7.5 [1M] - 1000 ml

Articolo #: 88027277

€ 85

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Shop Tris-HCl Buffer, pH 7.5 [1M] - 1000 ml online at a best price in Italia. B09V5L77RR
Peso del artículo2,2 libbre ( 1 kg )

DESCRIZIONE DEL PRODOTTO

Tris-HCl Buffer, pH 7.5 [1M] - 1000 ml

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Domande e risposte dei clienti

  • domanda: What is Tris-HCl Buffer, pH 7.5 used for?

    Rispondere: Tris-HCl Buffer, pH 7.5 is commonly used in biological and biochemical applications, particularly in DNA and RNA work. This buffer maintains a stable pH in various experiments, which is crucial for reactions such as PCR and enzyme assays. By providing a controlled environment for biomolecules, it ensures optimal conditions for enzymatic activity and stability.
  • domanda: Is Tris-HCl Buffer, pH 7.5 suitable for protein studies?

    Rispondere: Yes, Tris-HCl Buffer, pH 7.5 is frequently utilized in protein studies. Its buffering capacity is ideal for maintaining the pH during protein purification and electrophoresis. This stability helps prevent denaturation of proteins, making it an essential component in many laboratory protocols focused on protein analysis.
  • domanda: Can I use Tris-HCl Buffer for electrophoresis?

    Rispondere: Absolutely, Tris-HCl Buffer, pH 7.5 is widely used in gel electrophoresis, particularly for separating nucleic acids and proteins. The buffer's pH level optimizes the charge and behavior of biomolecules during the electrophoretic process, providing clearer separation and better resolution when visualizing the results.
  • domanda: What concentration is Tris-HCl Buffer, pH 7.5 provided in?

    Rispondere: Tris-HCl Buffer is offered in a 1M concentration, which means it contains one mole of Tris base per liter of solution. This high concentration is beneficial for a variety of experimental applications, allowing for precise buffering capabilities without significant dilution, thereby supporting effective results in biochemical applications.
  • domanda: How do I prepare Tris-HCl Buffer from powder?

    Rispondere: To prepare Tris-HCl Buffer, you would begin by dissolving Tris base in distilled water and adjusting the pH with hydrochloric acid. Typically, you will dissolve 121.14 grams of Tris in 800 ml of water, then add HCl to reach a pH of 7.5, and finally make up the volume to 1 liter. This method allows for tailored solutions based on specific experimental needs.
  • domanda: What is the role of pH in Tris-HCl Buffer?

    Rispondere: The pH of a buffer is critical as it affects the activity of enzymes and the stability of nucleic acids. Tris-HCl Buffer at pH 7.5 is particularly effective for biological experiments, as many enzymes, such as DNA polymerases, operate optimally around this pH. This creates a conducive environment for biochemical reactions, promoting efficient results.
  • domanda: How long can I store Tris-HCl Buffer, pH 7.5?

    Rispondere: Tris-HCl Buffer can typically be stored at room temperature away from direct sunlight for extended periods, up to several months, provided it is kept sterile and sealed. This longevity makes it a convenient buffer for repeated use in laboratory settings. Just ensure to check for any precipitate or color change before use.
  • domanda: Is Tris-HCl Buffer compatible with all biochemical applications?

    Rispondere: While Tris-HCl Buffer is versatile, it is not suitable for all applications, particularly those involving very acidic or basic conditions. It may interfere with certain chemical reactions in organic chemistry settings. Always consider the specific requirements of your experiment to ensure compatibility and optimal results.
  • domanda: Can Tris-HCl Buffer be used in molecular biology experiments?

    Rispondere: Yes, Tris-HCl Buffer, pH 7.5, is extensively utilized in molecular biology experiments. It is ideal for DNA and RNA work, such as in cloning, sequencing, and PCR. The buffer's stability at physiological pH allows for efficient handling and analysis of nucleic acids, greatly enhancing the quality of molecular biology research.
  • domanda: Where can I buy Tris-HCl Buffer, pH 7.5 [1M] - 1000 ml in Italy?

    Rispondere: You can purchase Tris-HCl Buffer, pH 7.5 [1M] - 1000 ml from Ubuy. They offer a variety of laboratory supplies, providing a convenient option for acquiring essential reagents like this buffer directly online.

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Professionisti

  • Stable pH for experiments
  • Fácil de usar
  • Applicazione versatile
  • Formulación de alta calidad
  • Well-reviewed by scientists

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  • El diseño de la botella podría mejorarse.

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  • Limitazioni: per i prodotti spediti a livello internazionale, si prega di notare che ogni garanzia del produttore potrebbe non essere valida, le opzioni di assistenza del produttore potrebbero non essere disponibili, i manuali del prodotto, le istruzioni e gli avvisi di sicurezza potrebbero non essere nella lingua del paese di destinazione. I prodotti (e i materiali di accompagnamento) potrebbero non essere progettati in conformità con gli standard, le specifiche e i requisiti di etichettatura del paese di destinazione e i prodotti potrebbero non essere conformi alla tensione di rete del paese di destinazione e ad altri standard elettrici (richiedendo l'uso di un adattatore o di un trasformatore, se necessario). Il destinatario è responsabile di assicurare che il prodotto possa essere legalmente importato nel paese di destinazione. Quando si ordina da Ubuy o dai suoi affiliati, il destinatario è l'importatore registrato ed è tenuto a rispettare tutte le leggi e i regolamenti del paese di destinazione.
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